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cell lines mouse embryonic stem cells  (ATCC)


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    ATCC cell lines mouse embryonic stem cells
    Cell Lines Mouse Embryonic Stem Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+es+cells/ES-E14TG2a/pm42247292-191-7-14
    Average 96 stars, based on 485 article reviews
    cell lines mouse embryonic stem cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture.
    Article Snippet: Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices.. It is difficult to employ pluripotent stem cells as resources for regenerative medicine due to risks of culture system contamination by animal-derived factors, or the large costs associated with the use of adhesive matrices.. To enable a coating-free culture system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment for polystyrene substrates, to improve adhesion and proliferation of pluripotent stem cells.

    Cell Culture:

    Article Title: UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture.
    Article Snippet: Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices.. It is difficult to employ pluripotent stem cells as resources for regenerative medicine due to risks of culture system contamination by animal-derived factors, or the large costs associated with the use of adhesive matrices.. To enable a coating-free culture system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment for polystyrene substrates, to improve adhesion and proliferation of pluripotent stem cells.

    Article Title: Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells
    Article Snippet: .. Mouse ES cells (ATCC) and iTS-P cells were maintained on feeder layers of mitomycin C-treated STO cells cultured in complete ES cell media containing 15% FBS (Millipore) as previously described – . ..

    Isolation:

    Article Title: PASK links cellular energy metabolism with a mitotic self-renewal network to establish differentiation competence
    Article Snippet: Cell line (Mouse) , C2C12 , ATCC , ATCC Cat# CRL-1772, RRID: CVCL_0188 , . .. Cell line (Mouse) , Mouse ES cells , ATCC , Isolated from mice , . .. Cell line (Mouse) , Primary mouse myoblasts , Isolated fresh from mice , Isolated from mice , .

    Mouse Assay:

    Article Title: PASK links cellular energy metabolism with a mitotic self-renewal network to establish differentiation competence
    Article Snippet: Cell line (Mouse) , C2C12 , ATCC , ATCC Cat# CRL-1772, RRID: CVCL_0188 , . .. Cell line (Mouse) , Mouse ES cells , ATCC , Isolated from mice , . .. Cell line (Mouse) , Primary mouse myoblasts , Isolated fresh from mice , Isolated from mice , .

    Stem Cell Culture:

    Article Title: A novel nested gene Aff3ir participates in vascular remodelling by enhancing endothelial cell differentiation in mice
    Article Snippet: .. Mouse ES cells (ESCs, ES-D3 cell line, CRL-1934) were purchased from ATCC and maintained in stem cell culture medium [DMEM (ATCC, Rockville, MD, USA) supplemented with 10ng/mL recombinant human leukaemia inhibitory factor (LIF; Chemicon, Temecula, CA, USA), 10% foetal bovine serum (FBS, ATCC), 0.1mmol/L 2-mercaptoethanol(2ME), 100U/mL penicillin, and 100μg/mL streptomycin] as describe previously . ..

    Recombinant:

    Article Title: A novel nested gene Aff3ir participates in vascular remodelling by enhancing endothelial cell differentiation in mice
    Article Snippet: .. Mouse ES cells (ESCs, ES-D3 cell line, CRL-1934) were purchased from ATCC and maintained in stem cell culture medium [DMEM (ATCC, Rockville, MD, USA) supplemented with 10ng/mL recombinant human leukaemia inhibitory factor (LIF; Chemicon, Temecula, CA, USA), 10% foetal bovine serum (FBS, ATCC), 0.1mmol/L 2-mercaptoethanol(2ME), 100U/mL penicillin, and 100μg/mL streptomycin] as describe previously . ..

    Knock-Out:

    Article Title: Rosa26 docking sites for investigating genetic circuit silencing in stem cells
    Article Snippet: The modified LTRi genetic switch (mLTRi) was constructed by cloning the transgene module into the vector module with DraIII and XhoI cut sites, and the individual modules were put together by Gibson assembly. mLTRi_EGFP is in process of being submitted to Addgene (addgene.org). .. Mouse ES cells (ATCC #SCRC-1023) were maintained in high glucose knockout DMEM (Life Technologies #10829 − 018) supplemented with 15% ES certified FBS (LifeTechnologies #10439024), 1% non-essential amino acids (Life Technologies #11140050), 1 mM l -glutamine (Life Technologies #25030 − 081), 0.1 mM 2-mercaptoethanol (Life Technologies #21985023) and 200 units/ml penicillin and streptomycin. .. The ES cells were plated on mitomycin C-treated mouse embryonic fibroblast cells (Millipore Sigma #PMEF-NL-P1) that are G418 resistant.

    Clinical Proteomics:

    Article Title: Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
    Article Snippet: .. To demonstrate the capability of the proposed optical system, we have characterized nucleic envelope and plasma membrane fluctuations in mouse ES cells, E14 (ATCC, CRL-1821). ..

    Membrane:

    Article Title: Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy
    Article Snippet: .. To demonstrate the capability of the proposed optical system, we have characterized nucleic envelope and plasma membrane fluctuations in mouse ES cells, E14 (ATCC, CRL-1821). ..



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    ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 <t>mouse</t> ES <t>cells</t> were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. <t>Live</t> cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.
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    ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 mouse ES cells were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. Live cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.

    Journal: bioRxiv

    Article Title: MCM10 and SLD-2/RECQL4 jointly activate the CMG helicase during metazoan DNA replication initiation

    doi: 10.64898/2026.04.01.715773

    Figure Lengend Snippet: ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 mouse ES cells were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. Live cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.

    Article Snippet: For the experiment in , live mouse ES cells were stained with a 4000 fold dilution of SPY650-DNA (SC501, Spirochrome), before imaging by spinning-disk confocal microscopy as described above for C. elegans .

    Techniques: Control, Incubation, High Content Screening, Microscopy, Immunostaining, Activation Assay, Western Blot, Phospho-proteomics, Staining, Binding Assay